C7642

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

FITC conjugate, Type IV, lyophilized powder

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

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Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
ApplicationFor the fluorescent detection of glycoproteins containing α−D-mannose or α−D-glucose
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
PackagingPackage size based on protein content
Physical formContains phosphate buffer salts and NaCl

Properties

typeType IV
formlyophilized powder
compositionProtein, ~10% biuret
extent of labeling3-6 mol FITC per mol protein
total impurities Carbohydrate, essentially free
conjugateFITC conjugate
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements42/43
Safety Statements22-36/37-45
WGK Germany3

References

referenceReeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521